SPRUCE Deep Peat Microbial Diversity, CO2 and CH4 Production in Response to Nutrient, Temperature, and pH Treatments during Incubation Studies
Description
This dataset contains the results of a microcosm incubation study on deep peat collected from the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experimental site within the Marcell Experimental Forest in northern Minnesota, USA in September 2014. Microcosms were monitored for carbon dioxide (CO2) and methane (CH4) production, and microbial community dynamics were assessed using quantitative polymerase chain reaction (qPCR) and amplicon sequencing.
The experiment was designed with a full factorial design with elevated temperature, nitrogen (N), (P), and pH treatments was used with samples from each transect serving replicates. In all, 96 microcosms were constructed to account for the 16 treatment combinations (Nitrogen x Phosphorous x pH x temperature), 2 time points, and 3 replicates. Temperature treatments were 6 degrees C, to mimic the SPRUCE ambient plot temperatures, and 15 degrees C to mimic the SPRUCE +9 degrees C treatment.
These data show that one year of deep peat warming, microbial community structure and abundance of bacterial, archaeal, fungal, and methanogenic populations showed strong vertical stratification across the peat depth profile yet no clear response to the temperature treatments (Kluber et al, 2020). In an effort to identify factors that may be limiting decomposition and microbial community change in deep peat, we conducted an anerobic microcosm incubation of deep peat (150-200 cm depth).
This dataset contains one data file in comma-separate values (*.csv) format. Additional metadata are provided: a data dictionary and a file-level metadata file in comma-separate values (.csv) format and a user guide in PDF (*.pdf) format.
Citation
This dataset is available for download at ESS-DIVE, please visit the DOI link in the citation below for access.
Kluber, LA, ER Johnston, SA Allen, JN Hendershot, PJ Hanson, CW Schadt. 2017. SPRUCE Deep Peat Microbial Diversity, CO2 and CH4 Production in Response to Nutrient, Temperature, and pH Treatments during Incubation Studies. ORNL Terrestrial Ecosystem Science SFA, ESS-DIVE repository. Dataset. https://doi.org/10.3334/CDIAC/spruce.040
Posted
Friday, May 19, 2017
SPRUCE ID: spruce.040
Public
