SPRUCE: Quantitative PCR (qPCR) of microbial gene copy numbers, 2021-2022
Description
This dataset provides the results for quantitative polymerase chain reaction (qPCR) of peat samples collected from ambient and experimental plots in the Spruce and Peatland Responses Under Climatic and Environmental Change (SPRUCE) experiment site in June and August of 2021, and June of 2022. SPRUCE is located within the Marcell Experimental Forest in northern Minnesota, USA. The dataset includes bacterial, archaeal, fungal gene copy numbers, along with corresponding logarithmic values, at 11 depth increments of two-meter deep peat cores taken from 12 sampling sites locations inside SPRUCE plots (10 chambered and 2 ambient plots). The sampling, sample prep and analysis followed standard methods outlined in prior publications (Wilson et al. 2016; Kluber et al. 2020) except that a higher yielding Omega Bio-Tek Mag-Bind Environmental DNA 96 Kit was used for extractions and DNA was quantified using Qubit dsDNA High Sensitivity Assay Kit. qPCR subsamples of peat cores from the SPRUCE plots characterize changes in the abundance and composition of microbial communities of peat seasonally showing how composition varies under multiple levels of experimental peat warming and atmospheric CO2 concentrations.
This dataset contains one data file in comma-separated values (.csv) format. Additional metadata are provided: one data dictionary and a file-level metadata file in comma-separated values (.csv) format and a user guide in PDF (*.pdf) format.
On 2026-07-07 this dataset was updated to add three columns to the data file: ‘Fungal_copy_dry’, ‘Log_fungal_copy_dry’, ‘Fungal_copy_wet’. No previously released data values were altered. Additionally, the abstract, data dictionary, and user guide were updated, and a file-level metadata file was added.
Citation
This dataset is available for download at ESS-DIVE, please visit the DOI link in the citation below for access.
Roth, S, Z Buell, B Kristy, J Philips, S Ottinger, K Lowe, MA Mayes, and CW Schadt. 2024. SPRUCE Quantitative PCR of Microbial Gene Copy Numbers, 2021-2022. ORNL Terrestrial Ecosystem Science SFA, ESS-DIVE repository. Dataset. https://doi.org/10.25581/spruce.110/1998878
Posted
Friday, January 26, 2024
SPRUCE ID: spruce.110
Public
