@article{910,
  keywords = {Ecology, Evolution, Behavior and Systematics, Microbiology},
  author = {Alyssa Carrell and Dušan Veličković and Travis Lawrence and Benjamin Bowen and Katherine Louie and Dana Carper and Rosalie Chu and Hugh Mitchell and Galya Orr and Lye Markillie and Sara Jawdy and Jane Grimwood and Jonathan Shaw and Jeremy Schmutz and Trent Northen and Christopher Anderton and Dale Pelletier and David Weston},
  title = {Novel metabolic interactions and environmental conditions mediate the boreal peatmoss-cyanobacteria mutualism},
  abstract = {<jats:title>Abstract</jats:title><jats:p>Interactions between <jats:italic>Sphagnum</jats:italic> (peat moss) and cyanobacteria play critical roles in terrestrial carbon and nitrogen cycling processes. Knowledge of the metabolites exchanged, the physiological processes involved, and the environmental conditions allowing the formation of symbiosis is important for a better understanding of the mechanisms underlying these interactions. In this study, we used a cross-feeding approach with spatially resolved metabolite profiling and metatranscriptomics to characterize the symbiosis between <jats:italic>Sphagnum</jats:italic> and <jats:italic>Nostoc</jats:italic> cyanobacteria. A pH gradient study revealed that the <jats:italic>Sphagnum–Nostoc</jats:italic> symbiosis was driven by pH, with mutualism occurring only at low pH. Metabolic cross-feeding studies along with spatially resolved matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) identified trehalose as the main carbohydrate source released by <jats:italic>Sphagnum</jats:italic>, which were depleted by <jats:italic>Nostoc</jats:italic> along with sulfur-containing choline-O-sulfate, taurine and sulfoacetate. In exchange, <jats:italic>Nostoc</jats:italic> increased exudation of purines and amino acids. Metatranscriptome analysis indicated that <jats:italic>Sphagnum</jats:italic> host defense was downregulated when in direct contact with the <jats:italic>Nostoc</jats:italic> symbiont, but not as a result of chemical contact alone. The observations in this study elucidated environmental, metabolic, and physiological underpinnings of the widespread plant–cyanobacterial symbioses with important implications for predicting carbon and nitrogen cycling in peatland ecosystems as well as the basis of general host-microbe interactions.</jats:p>},
  year = {2021},
  booktitle = {The ISME Journal},
  journal = {The ISME Journal},
  series = {The ISME Journal},
  volume = {16},
  pages = {1074-1085},
  institution = {Springer Science and Business Media LLC},
  organization = {Springer Science and Business Media LLC},
  publisher = {Springer Science and Business Media LLC},
  school = {Springer Science and Business Media LLC},
  issn = {1751-7362, 1751-7370},
  doi = {10.1038/s41396-021-01136-0},
  crossref = {},
  editor = {},
}