SPRUCE Soil Metabolome Responses to Whole Ecosystem Warming in SPRUCE Experimental Plots, August 2016

This dataset reports a suite of complementary environmental geochemical analyses of peat and pore water samples from SPRUCE experimental plots across a range of whole ecosystem warming (WEW) temperature treatments collected in August of 2016. Results of analyses of peat extracts include NMR and GC-MS analyses of central metabolites, liquid chromatography mass spectra (LC-MS) analyses of lipids, and quantitative proteomics. Pore water was analyzed by FTICR-MS for secondary metabolites and for carbon dioxide (CO2), methane (CH4) concentrations, stable isotope composition, and the differential solubility corrected CO2:CH4 ratio.
Analyses show that WEW stimulated primary production in warmer treatment enclosures resulting in increased fresh, labile organic matter inputs to the surface peat, thereby enhancing microbial activity and greenhouse gas production.
Investigations leveraged the Spruce and Peatland Responses Under Changing Environments (SPRUCE) experiment where air and peat warming were combined in WEW treatments. SPRUCE is located at the 8.1-ha S1 Bog forest site in northern Minnesota, 40 km north of Grand Rapids, in the USDA Forest Service Marcell Experimental Forest (MEF).