SPRUCE: Quantitative PCR (qPCR) of microbial gene copy numbers, 2021-2022

Description

This data set provides the results for quantitative polymerase chain reaction (qPCR) of peat samples collected from ambient and experimental plots in the Spruce and Peatland Responses Under Climatic and Environmental Change (SPRUCE) experiment site in June and August of 2021, and June of 2022. Additional dates and fungal gene copy data are in progress and will be added to the dataset when available. The data set includes bacterial and archaeal gene copy numbers, along with corresponding logarithmic values, at 11 depth increments of two-meter deep peat cores taken from 12 sampling sites locations inside SPRUCE plots(10 chambered and  2 ambient plots). The sampling, sample prep and analysis followed standard methods outlined in prior publications (Wilson et al. 2016; Kluber et al. 2020) except that a higher yielding Omega Bio-Tek Mag-Bind Environmental DNA 96 Kit was used for extractions and DNA was quantified using Qubit dsDNA High Sensitivity Assay Kit. This data set contains one file in comma separate (*.csv) format. 

Citation

Roth, S., Z. Buell, B. Kristy, J. Philips, S. Ottinger, K. Lowe, M.A. Mayes, & C.W. Schadt. 2024. SPRUCE Quantitative PCR of microbial gene copy numbers, 2021-2022. Oak Ridge National Laboratory, TES SFA, U.S. Department of Energy, Oak Ridge, Tennessee, U.S.A. https://doi.org/10.25581/spruce.110/1998878.

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Friday, January 26, 2024

SPRUCE ID: spruce.110

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